mRNA in rat brain: pharmacological manipulation
نویسندگان
چکیده
Using in situ hybridization and immunohistochemical techniques, we examined the expression pattern of egr-1 mRNA and Egr-1 Ž . protein in several brain regions following administration of 3,4-methylenedioxymethamphetamine MDMA . Furthermore, we also Ž . Ž . studied the role of N-methyl-D-aspartate NMDA receptor, dopamine D receptor, 5-hydroxytryptamine 5-HT transporter or 5-HT 1 2A receptor in the induction of egr-1 mRNA by MDMA. Basal constitutive levels of egr-1 mRNA were detected in control rat brains. A Ž . single administration of MDMA 10 mgrkg caused marked induction of egr-1 mRNA in the prefrontal cortex, striatum and hippocampal dentate gyrus. However, no changes in the egr-1 mRNA levels were detected in the CA1 region of hippocampus and occipital cortex after Ž . administration of MDMA 10 mgrkg . Furthermore, the expression of egr-1 mRNA in the prefrontal cortex, striatum and hippocampal Ž . dentate gyrus after administration of MDMA 10 mgrkg was blocked significantly by pretreatment with NMDA receptor antagonist Ž . Ž . w x ŽŽ . . 5R,10S q -5-methyl-10,11-dihydro-5H-dibenzo a,b -cyclohepten-5,10-imine q -MK801; 1 mgrkg , dopamine D receptor antago1 Ž . Ž . Ž nist SCH 23390 1 mgrkg or 5-HT uptake inhibitor paroxetine 5 mgrkg , but not by 5-HT receptor antagonist SR46349B 5 2A . Ž mgrkg . However, high basal levels of Egr-1 immunoreactivity in the rat brain were not altered by administration of MDMA 10 . mgrkg . These results suggest that MDMA alters the expression of egr-1 mRNA in several regions of rat brain, and that the expression of egr-1 mRNA by MDMA in the prefrontal cortex, striatum and hippocampal dentate gyrus appears to be mediated, at least in part, by NMDA receptor, dopamine D receptor and 5-HT transporter. q 2000 Elsevier Science B.V. All rights reserved. 1 Ž . Ž . Ž .
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